2026 IHCIF Schedule

PRE-ANALYTICS

Stephen Hewitt

Lecture 1: Pre-Analytics I: Total Test and Endogenous Variables

Lecture 2: Pre-Analytics II: Exogenous Variables and Preservation

Lecture 3: Pre-Analytics III: Tissue Processing, Microtomy and Specimen Storage

FUNDAMENTALS OF LABELS and STAINS

Paul Goodwin

Lecture 1: Fundamentals of Light

Lecture 2: Exploiting Intensity

Lecture 3: Exploiting Wavelength

Basics of Microscopy 

Paul Goodwin 

Lecture 1: Transmitted Light Microscopy 

Lecture 2: Fluoresence Microscopy 

Lacture 3: Limitations of Microscopy 

FUNDAMENTALS OF IMMUNOHISTOCHEMISTRY (IHC)

Lecture 1: Antibody Basics for IHC

Frank Peale (25 mins) 

Lecture 2: Protocol validation Part 1: Controls for immunohohistochemistry

Jody Longo (25 mins) 

Hands-On Microscope Time (Paul Goodwin & Russell Ulbrich) & Introduction to IF lab with organoids (Liz Fisher)

3 groups, rotating 

Part one of Fluorsence Module (Liz Fisher, Kate Tubbesing, Margarida Barroso)

Perm/ block and primaries 

ADVANCED IHC CONCEPTS Part 1

Lecture 1: Antigen Retrieval

Jody Longo 

Lecture 2: Autofluorescence 

Liz Fisher 

ADVANCED IHC CONCEPTS Part 2

Lecture 3: Multiplex Immunohistochemistry  -Frank Peale 

 Lecture 4: Alternative In Situ Assays and Detection Strategies-Micheal Gerdes 

Lecture 5: Trouble Shooting the Specificity of Molecular Target -Kate Tubbesing

Candle House Lecture Space 

Coffee/ drinks available 

Part two of Fluoresence lab (Liz Fisher, Kate Tubbesing, Margarida Barroso) 

Secondarys, direct congugates, DAPI 

practice coversliping in groups during incubations (all faculty welcome)

Coverslip organoids 

Discussions/ instruction in small groups during incubations 

160 Lab Space 

break-out room available for coffee/ beverages 

IHC/ Chromagen Module Part 1 ( Longo. Peale, Gerdes as leads) 

Lab introduction - model explained, protocols described 

Processing, Antigen retrieval (explain alternatives), perm/ block 

Primary incubation started 

160 Lab Space 

break-out room available for coffee/ beverages 

IHC/ Chromagen Module part 2  & Microscopy Time (groups rotate) 

HRP/ AP processing 

160 Lab Space 

break-out room available for coffee/ beverages 

Some in imaging core 

Image Focus Lectures 

Lecture 1 : Image archiving, management and forensics- Longo

lecture 2: Images and Figures in Publication - Barroso

IHC/ Chromagen Module part 2  & Microscopy Time (groups rotate) 

light sheet demo ** 

Extra processing or Imaging Time - set up tissuefaxs for overnight running of samples 

Advanced Approaches and Quantifiaction (45 mins each) 

Lecture 1: Super-resolution microscopy-  Goodwin 

Lecture 2: FRET and advanced quantification -Barroso

IHC Imaging

Extra IF Imaging 

Small groups - Open images and generate montages or figures 

160 Lab space 

Imaging Core 

Advanced Approaches and Quantifiaction  Part 2 (45 mins each) 

Frank Peale : Quantifiable Controls for IHC 

Kate Tubbesing : Analysis Tools and use of AI/ML

lab wrap-up

Final chance for imaging core oppurtunites

Work in small groups with ImageJ or other programs to generate montage 

send favorite images collected with description for class of 2026 ppt

lab 160

imaging core

Advanced Approaches and Quantifiaction  Part 2 (45 mins each) 

Multiplexing IF -  Gerdes 

Open lecture topic (TBA)

Bio-imaging Ethics - 

talk (TBA) and panel discussion - students bring questions