Back to Resources & Research Facilities Imaging Resources Overview Microscopes in Development Home Groundbreaking Research at the MBL Resources & Research Facilities Imaging Resources Microscopes in Development Dual-view Line Scanning Confocal General Overview: A custom-built line scanning confocal microscope with two opposing views that is capable of imaging samples up to several millimeters in length at high resolution and fast speed. Commercial or Pre-commercial: Pre-Commercial Which Facility: Imaging Innovation Initiative Imaging Mode: Optical If Optical: Confocal Microscope Stand: 4-pi configuration custom designed Features: Capable of imaging large areas and volumes rapidly with high NA objective lenses. Simultaneous two-sided illumination and detection to increase volumetric speed and obtain sample information that may be occluded from a single view. Laser Wavelengths: 405 nm, 488 nm, 561 nm, 640 nm Excitation/Emission Filters: Quad band filter 405/488/561/640 lasers. A filter wheel with an individual band bass filter for each laser excitation wavelength. Detector: Camera Imaging lenses: 25x, 1.1 NA Water Immersion 40x, 1.25 NA Silicone 60x, 1.4 NA Oil 100x, 1.35 NA Silicone 100x, 1.49 NA Oil Microscope Control software: Custom designed Graphical User Interface in Matlab Image Analysis Software: Custom designed in Matlab CO2: No Humidity: No Light Tight: Yes Sample mounting requirements/constraints: Convention sample mounting approaches. Glass Bottom Petridish Location - Building and Room: Lillie 219 Contact Person: Matthew Parent Total Internal Reflection Fluorescence (TIRF) Microscope General Overview: Home-built TIRF Microscope capable of illuminating samples in a ring-TIR mode or a single sided TIR. Commercial or Pre-commercial: Pre-Commercial Which Facility: Imaging Innovation Initiative Imaging Mode: Optical If Optical: Widefield/TIRF Microscope Stand: Inverted Features: Epi illumination or TIR illumination Laser Wavelengths: 405 nm, 488 nm, 561 nm, 640 nm Excitation/Emission Filters: Quad band filter 405/488/561/640 lasers. Individual emission filters can be added manually. Detector: Camera Imaging lenses: 100x, 1.49 NA TIRF lens Microscope Control software: Custom designed Graphical User Interface in Matlab and u-Manager Image Analysis Software: CO2: No Humidity: No Light Tight: Yes Sample mounting requirements/constraints: Conventional sample mounting. Location - Building and Room: Lillie 219 Hourly Rate for Use: $25.00 Contact Person: Matthew Parent Dual-view Selective Plane Illumination Microscope using multi-immersion lenses General Overview: A home built dual-view SPIM system using multi-immersion lenses for imaging clear brain tissues. This system is also suitable for imaging samples in water/sea water. Commercial or Pre-commercial: Pre-Commercial Which Facility: Imaging Innovation Initiative Imaging Mode: Optical If Optical: Lightsheet Microscope Stand: Custom designed frame Features: Suitable for imaging large samples at high volumetric speed. Laser Wavelengths: 405 nm, 488 nm, 561 nm, 640 nm Excitation/Emission Filters: Quad band filter for 405/488/561/640 lasers. Detector: Camera Imaging lenses: 14x, 0.4 NA Multi-immersion lenses 23x, 0.7 NA Multi-immersion lenses Microscope Control software: Custom designed Graphical User Interface in Matlab Image Analysis Software: Fiji/Matlab CO2: No Humidity: No Light Tight: No Sample mounting requirements/constraints: Conventional sample mounting Location - Building and Room: Lillie 219 Contact Person: Matthew Parent Anisotropy Imaging System General Overview: This system facilitates illuminating samples using a linearly polarized light in Epi or TIRF mode and detects fluorescence emission in parallel and perpendicular directions using a single sCMOS camera. Anisotropy imaging can provide information about protein-protein interaction or protein clustering. Commercial or Pre-commercial: Pre-Commercial Which Facility: Imaging Innovation Initiative Imaging Mode: Optical If Optical: Widefield/TIRF Microscope Stand: Inverted Features: 405 nm, 488 nm, 561 nm, 640 nm Laser Wavelengths: A single band pass filter appropriate for the illumination. Detector: Camera Imaging lenses: 100x, 1.49 NA Oil TIRF lens 60x, 1.4 NA Oil lens Microscope Control software: Custom designed Graphical User Interface in Matlab Image Analysis Software: Custom designed Image Analysis Graphical User Interface in Matlab CO2: No Humidity: No Light Tight: Yes Sample mounting requirements/constraints: Conventional sample mounting Location - Building and Room: Lillie 219 Contact Person: Matthew Parent