Back to Education High School Science Discovery Program Overview Frequently Asked Questions Participating Schools Sample Course Schedule Student Experiences Home Educational Opportunities High School Science Discovery Program Sample Course Schedule CRISPR/Cas9 Genome Editing: A Hands-on Experience Time Wednesday Thursday Friday Saturday 7:00 Breakfast 7 - 8:30 am Breakfast 7 - 8:00 am Breakfast 7 - 8:00 am 7:30 8:00 8:30 IT Session - Meigs: Access to network and shared drives Check embryos 8:00 - 8:30 am Check embryos 8:00 - 8:30 am 9:00 Lab - Loeb 207: Injections of gRNAs and dyes, 9:00 am - 11:30 pm Lab - Loeb 207: Injections, Score Phenotypes, dechorinate, fix and do phalloidin and dapi stain 9:00 am - 12:00 pm Lab - Loeb 207: score second round of phenotypes, wrap up discussion about phenotypes and CRISPR, discussion on ethics and CRISPR. Archiv's tour and Gift shop time 9:00 am - 12:00 pm 9:30 10:00 Arrival & Check-In: 10:00 am - 12:00 pm 10:30 11:00 11:30 Tour - MRC tank room 11:30 am - 12:00 pm 12:00 Lunch Noon - 1:00 Lunch - Noon to 1:30 pm Lunch - Noon to 1:30 pm Lunch - Noon to 1:30 pm 12:30 1:00 MBL Orientation & Safety Training Loeb 263 1:30 Lab - Loeb 207: Intro to Imaging competition. Intro to fluorescent imaging, GFP Lines, Fluorescent Staining, maybe hair cell staining & Time Lapse Development 1:30 - 5:00 pm Lab - Loeb 207: Image fixed, stained animals 1:30 - 5:00 pm Departure & Check-Out: 1:30 pm 2:00 Lecture - Loeb 263: Introduction to embryology & development & overview of how CRISPR works 2:30 3:00 Lab - Loeb 207: Introduction to Microscopes and learn how to inject 3:30 4:00 4:30 5:00 Dinner 5:30 to 6:30 pm Pizza & Movie Night beginning at 6 pm, Meigs Room, Swope Center Dinner 5:30 to 6:30 pm 5:30 6:00 6:30 Work on images for image competition, submit by 10pm 7:00 7:30