Thurs 23 Jul

2:00 – 5:00 Student check-in (Swope)

6:00 – 7:00 Dinner (Swope)

7:00 – 7:15 Welcome to the Zebrafish Course: Tom Schilling & Andi Pauli (Speck Auditorium)

7:15 – 8:15 Overview: Zebrafish Development and Genetics: Lila Solnica-Krezel (Speck Auditorium)

8:15 – 9:15 Student 3-min oral presentations (Speck Auditorium)

9:30 – 10:30 Set up posters and locate individual student stations in lab (Loeb 260)

Fri 24 Jul

8:45 – 9:00 Welcome to the MBL: Linda Hyman, Director of Education (Loeb 260)

9:00 – 9:30 Introduction to stereoscopes, iPad/camera & server: Dave Raible & Faculty (Loeb 260)

9:30 – 12:00 Pit Talk/Lab: Early Dev: Lila Solnica-Krezel, Margot Williams & Dave Raible (Loeb 260)

12:00 – 1:30 Faculty (Tom Schilling & Andi Pauli) lunch with students (Swope)

1:30 – 2:30 Lab: Early Zfish Dev, continued: L Solnica-Krezel, M Williams & D Raible (Loeb 260)

2:30 – 3:30 Lecture: Introduction to Microscopy: Andres Collazo (Loeb 263)

3:30 – 6:00 Hands-on microscopy training: Andres Collazo, Jim Fadool, Dave Raible, & T Schilling (Loeb 260)

6:00 – 7:30 Dinner (Swope)

7:30 – 9:00 Research Lectures & Discussion: Margot Williams & Mary Mullins (Speck Auditorium)

9:00 – 9:30 Follow up on embryos and wrap-up from morning session: Lila Solnica-Krezel

9:30 – 11:00 ‘DiSci’ (science discussions with speakers, faculty, posters 1-6 on display)

Sat 25 Jul

8:30 – 9:30 Pit Talk: Larval anatomy: Tom Schilling (Loeb 260)

9:30 – 12:00 Lab: Larval anatomy tour with fluorescent transgenics: Andres Collazo, Jim Fadool, Dave Raible & Tom Schilling (Loeb 260)

12:00 – 1:30 Faculty (Mary Mullins & Lila Solnica-Krezel) lunch with students (Swope)

1:30 – 2:30 Workshop: Developmental Genetics: Mary Mullins (Candle House 104)

2:30 – 3:30 Workshop: Testing Gene Function & Designer Genes: Mary Mullins (Candle House 104)

3:30 – 4:30 Lab: imaging and mounting transgenics: Dave Raible & Tom Schilling (Loeb 260)

4:30- 5:00 Break

5:00 – 6:00 Lecture: Light Sheet Microscopy: Jan Huisken (Speck Auditorium)

6:00 – 7:30 Dinner (Swope)

7:30 – 9:00 Research Lectures & Discussion: Lila Solnica-Krezel & Dave Raible (Speck Auditorium)

9:00 – 11:00 ‘DiSci’ (continued science discussions with speakers, faculty, posters 7-12)

9:30 – 11:00 Time-lapse microscopy: Andres Collazo & Jim Fadool (Group 1)

9:30 – 11:00 Time-lapse microscopy: Jan Huisken and Dane Maxfield (Group 4)

9:30 – 11:00 Groups 2 and 3 work on T-shirt design

Sun 26 Jul

8:30 – 9:30 Wrap-up: Larval Anatomy: Tom Schilling & Dave Raible (Loeb 263)

9:30 – 12:00 Pit Talk & Lab: Mounting, imaging & analysis of mRNA and protein in embryos and larvae: Kara Cerveny & Fabienne Poulain (Loeb 260)

12:00 – 1:30 Faculty (Dave Raible & Margot Williams) lunch with students (Swope)

1:30 – 4:00 Lab: Mounting, imaging, & analysis of mRNA and protein, including wrap up: Kara Cerveny & Fabienne Poulain (Loeb 260)

4:00 - 4:30 Break

4:30 – 6:00 Time-lapse microscopy: Andres Collazo & Jim Fadool (Group 2)

4:30 – 6:00 Time-lapse microscopy: Jan Huisken and Dane Maxfield (Group 3)

4:30 – 6:00 Groups 1 and 4 work on T-shirt design

6:00 – 7:30 Dinner (Swope)

7:30 – 9:00 Research Lectures & Discussion: Jim Fadool & Fabienne Poulaine (Speck Auditorium)

9:00 – 11:00 ‘DiSci’ (continued w speakers and all faculty present, posters 13-19) + T shirt design

Mon 27 Jul

8:30 – 12:00 Pit Talk & Lab: mRNA and CRISPR injections: Andi Pauli (Loeb 260)

12:00 – 1:30 Faculty (Fabienne Poulain & Kara Cerveny) lunch with students (Swope)

1:30 – 3:00 FREE time

3:00 – 4:30 Workshop: Responsible conduct of research: Tom Schilling & Andi Pauli (Candle House 104, or Loeb quad)

4:30 – 6:00 Time-lapse microscopy: Andres Collazo & Jim Fadool (Group 3)

4:30 – 6:00 Time-lapse microscopy: Jan Huisken and Dane Maxfield (Group 1)

4:30 – 6:00 Groups 2 and 4 work on T-shirt design

6:00 – 7:30 Dinner (Swope)

7:30 – 9:00 Research Lectures & Discussion: Kara Cerveny & Holger Knaut (Speck Auditorium)

9:00 – 9:45 Wrap-up mRNA injected embryos: Andi Pauli (Loeb 260); start 2 time-lapses with germline-labelled or fluorescently injected embryos

9:45 – 11:00 ‘DiSci’ (continued w speakers and all faculty present, posters 19-24)

11:00 T-shirt design is due by 11:00 pm

Tues 28 Jul

8:30 – 12:00 Pit Talk & Lab: Morpholino & CRISPR injections: Andi Pauli & Jeff/Holger (Starr 209, then Loeb 260)

11:00 – 12:00 Needle pulling demo: Adair Oesterle and Jim Fadool (Loeb 260)

12:00 – 1:30 Faculty (Jeff Farrell & Jan Huisken) lunch with students (Swope)

1:30 – 4:00 Workshop: Genome and genomics literacy: Jeff Farrell (Bay Reading Room in Lillie)

4:00 – 5:30 Workshop: Single-cell and Spatial Transcriptomics: Alex Schier (Candle House 104)

5:30 – 6:00 Check on mRNA injected embryos: Andi Pauli & Jeff/Holger (Loeb 260)

6:30 – 7:30 Student/Faculty Challenge: Scavenger Hunt

7:30 – 10:00 Course BBQ (Loeb Quad)

10:00 – 11:30 Time-lapse microscopy: Andres Collazo & Jim Fadool (Group 4) (and any good ones from the morning’s injected larvae)

10:00 – 11:30 Time-lapse microscopy: Jan Huisken and Dane Maxfield (Group 2) (and any good ones from the morning’s injected larvae)

Wed 29 Jul

9:00 – 10:30 Workshop: Zebrafish in Education: Monica Blatnik, Pierre Le Pabic & Kara Cerveny (Starr 209)

10:30 – 12:00 Pit Talk: Chimeric Analysis: Tom Schilling, Holger Knaut & Jim Fadool (Loeb 260)

12:00 – 1:30 Faculty (Alex Schier & Andres Collazo) lunch with students (Swope)

1:30 – 5:30 Lab: Chimeric analysis – Blastula transplants: Andreas Collazo, Jim Fadool, Holger Knaut & Tom Schilling (Loeb 260)

from 5:30 on Student self-organized social time and/or assembly of images – free evening!

6:00 – 7:00 Faculty Meeting (Memorial Circle)

7:00 – 10:00 Faculty & Staff Event

Thurs 30 Jul

8:30 – 9:30 Workshop: When should I (not) use zebrafish as a model? Alex Schier (Starr 209)

9:30 – 12:00 Lab: Imaging transplants: Andres Collazo, Jim Fadool, Holger Knaut & Tom Schilling (Loeb 260)

12:00 – 1:00 Faculty (Holger Knaut & Jim Fadool) lunch with students (Swope)

1:00 – 4:30 Pit Talk & Lab: Adult dissections & tissue regeneration: Mayssa Mokalled (Loeb 260)

4:30 – 6:30 Workshop: Mutant & CRISPR design: Jeff Farrell (Bay Reading Room in Lillie)

6:30 – 7:30 Dinner (Swope)

7:30 – 9:00 Research Lectures & Discussion: The Ruth Sager Lectures in Genetics:

Jeff Farrell & Alex Schier (Speck Auditorium)

9:00 – 11:00 ‘DiSci’ (continued w speakers and all faculty present, posters 1-6)

9:30 – 11:00 Additional imaging of transplants; assembly of images for transplant wrap-up

Fri 31 Jul

***Work on photo contest entries during ‘free time’ today***

8:30 – 10:30 Wrap-up: Chimeric analysis student presentations: Andres Collazo, Jim Fadool, Holger Knaut & Tom Schilling (Starr 209)

10:30 – 12:00 Review of time-lapses (Groups 1, 2, 3, 4): Jim Fadool, Andres Collazo & Jan Huisken (Starr 209)

12:00 – 1:30 Faculty (Corinne Houart & Monica Blatnik) lunch with students (Swope) (Groups 1 and 2: lunch till 1pm)

1:30 – 3:00 Workshop: Disease modeling: Martin Distel (Candle House 104)

3:00 – 5:00 FREE TIME/work on photo-contest/tours MRC

Tour MRC groups 1&2 and 3&4

5:00 – 6:00 Research Lecture & Discussion: Mayssa Mokalled (Speck Auditorium)

6:00 – 7:00 Dinner (Swope)

8:00 – 9:00 MBL Friday evening lecture (Clapp Auditorium)

9:00 – 11:00 ‘DiSci’ (continued w speakers and all faculty present, posters 7-12)

Sat 1 Aug

***Work on photo contest entries during ‘free time’ today***

8:30 – 12:00 Pit talk & Lab: Disease models: Martin Distel (Loeb 260)

12:00 – 1:00 Faculty (Mayssa Mokalled & Pierre Le Pabic) lunch with students (Swope)

1:00 – 2:00 Pit Talk: Monitoring morphogenesis: Analysis of morphogenesis in 3D and 4D: Kristen Kwan (Loeb 263)

2:30 – 5:00 Lab: Monitoring morphogenesis: Kristen Kwan (Loeb 260)

5:00 – 6:00 Wrap-up of Monitoring morphogenesis: Kristen Kwan (Loeb 263)

6:00 – 7:30 Dinner (Swope)

7:30 – 9:00 Research Lectures & Discussion: Martin Distel & Kristen Kwan (Speck Auditorium)

9:00 – 11:00 ‘DiSci’ (continued w speakers and all faculty present, posters 13-19)

9:30 – 11:00 Free Time prep for wrap up

9:30 – 10:30 Time-lapse microscopy: Jim Fadool, Paul, Andres & Dane (all groups)

Sun 2 Aug

***Work on five-minute student presentations of favorite data during ‘free time’ today***

8:30 – 9:30 Pit Talk: Behavior: Harry Burgess & Jim Fadool (Starr 209)

9:30 – 12:00 Lab: Behavior: Harry Burgess & Jim Fadool & John Dowling (Loeb 260)

12:00 – 1:30 Faculty (Kristen Kwan & Martin Distel) lunch with students (Swope)

1:30 – 4:00 Lab, continued: Behavior: Harry Burgess & Jim Fadool & John Dowling (Loeb 260)

4:00 – 5:00 Wrap-up of Behavior: Harry Burgess & Jim Fadool & John Dowling (Loeb 260)

5:00 – 6:00 Workshop: Transgenesis: Sarah Kucenas (Candle House 104)

6:00 – 7:30 Dinner (Swope)

7:30 – 9:00 Chi-Bin Chien Memorial Research Lecture: Jamie Nichols (Speck Auditorium), followed by reception (Loeb Quad or MBL Club)

9:00 – 11:00 ‘DiSci’ (continued w speakers and all faculty present, posters 20-24)

10:00 Stony Beach evening quiet field trip to see bioluminescence (weather permitting)

Mon 3 Aug

***Work on five-minute student presentations of favorite data during ‘free time’ today***

8:30 – 9:30 Pit Talk: Optical Manipulation: Sarah Kucenas & Harry Burgess (Loeb 263)

9:30 – 12:00 Lab Rotations: Ablation/Axotomy & Optogenetics: Sarah Kucenas & Harry Burgess (Loeb 260) AND *free time*

12:00 – 1:00 Faculty (Jamie Nichols) lunch with students (Swope)

1:00 – 4:00 Lab Rotations: Ablation/Axotomy & Optogenetics: Sarah Kucenas & Harry Burgess (Loeb 260) AND Fish facility tours with aquaculture vendors

4:00 – 4:45 Tea-time with aquaculture vendors (Loeb Quad or MBL Club)

4:45 – 6:00 Work on five-minute student presentations of favorite data

6:00 Photo contest images (individual & group entries) due by 6 pm 

6:00 – 7:30 Dinner (Swope)

7:30 – 9:00 Research Lectures & Discussion: Sarah Kucenas & Harry Burgess (Speck Auditorium)

9:00 – 9:30 ‘DiSci’ (continued w speakers and all faculty present)

9:30 – 10:30 Follow up on Ablation/Axotomy & Optogenetics: Sarah Kucenas & Harry Burgess (Candle House 104)

10:30 Stony Beach evening quiet field trip to see bioluminescence (weather backup)

Tues 4 Aug

***Work on five-minute student presentations of favorite data during ‘free time’ today***

9:00 – 11:00 Lab: Disease modeling follow-up: Martin Distel (Loeb 260)

11:00 – 12:00 Wrap-up: Disease modeling (Martin Distel) (Loeb 263)

12:00 – 1:30 Faculty (Harry Burgess & Sarah Kucenas) lunch with students (Swope)

1:30 – 5:00 Lab: Monitoring tissue regeneration: Mayssa Mokalled (Loeb 260)

5:00 – 6:00 Wrap-up: Monitoring tissue regeneration: Mayssa Mokalled (Loeb 263)

6:00 – 7:30 Dinner (Swope)

7:30 – 9:00 Research Lectures & Discussion: Andi Pauli & Tom Schilling (Speck Auditorium)

9:00 – 9:30 ‘DiSci’ (continued w speakers and all faculty present)

9:30 – 11 Finish work on five-minute student presentations of favorite data & photo contest, if needed

11:00 Late-breaking photo contest images due by 11:00 pm 

Wed 5 Aug

9:00 – 10:30 Lab: In vitro fertilization: April Freeman & Andi Pauli (Loeb 260)

10:30 – 12:00 Workshop: Zebrafish Husbandry, Breeding and Shipping: April Freeman (Loeb 263)

12:00 – 1:30 Faculty (April Freeman & Caitlin Inglis & Troy McInerney) lunch with students (Swope)

1:30 – 3:30 Five-minute student presentations of favorite data (Candle House 104)

3:30 – 5:00 Course Wrap-up Discussion: Tom Schilling & Andi Pauli (Tent in Loeb Quad)

5:00 – 5:15 Course Photo #1; wear course t-shirt (Loeb Courtyard)

6:00 – 10:00 Course Dinner and Student Awards with Course Photo #2 at 6:30 pm (Swope Lower Terrace)

Thurs 6 Aug

Breakfast, room checkout and departure