Xla.Tg(CMV:Brainbow-1.0H)NXR

NXR_0001

Description: (Brainbow H) Default state is ubiquitous whole cell red fluorescence. Following Cre induced recombination cells will have membrane yellow or membrane cyan fluorescence.

Reference: Nature. 2007 Nov;450:56-62Xenbase

Xla.Tg(CMV:Brainbow-1.0L)NXR

NXR_0002

Description: (BrainbowL) Default state is ubiquitous whole cell red fluorescence. Following Cre induced recombination cells will have whole cell yellow or whole cell cyan fluorescence.

Reference: Nature. 2007 Nov;450:56-62Xenbase.

brainbow1

Xla.Tg(LCMV:eYFP,eCFP)Ryff

NXR_0003

Description: (C5) Whole embryo blue fluorescence; yellow fluorescence upon Cre or FLP recombination.

Reference: Nucleic Acids Res. 2003 Apr;31:e44 | Dev Dyn. 2006 Aug;235:2220-8XenbaseEXRC

LCMVECFPloxPFRTEYFPC5.2-1024x767

Xla.Tg(CAG:GFP)Mead

NXR_0004

Description: Fluorescence detectable in most tissues through development.

Available as frozen sperm, please allow 8 months for live animals.

Reference: Mead Lab. Xenbase

CAG-GFP

NXR_0006

Description: (CAG-venus(YFP) LineB) pCAGGS driving Venus YFP expression under the control of the CAG promoter and is followed by the rabbit β-globin polyA signal sequence.

Reference: Nat Biotechnol. 2002 Jan;20:87-90 | Dev Dyn. 2005 Jun;233:562-9XenbaseEXRC

CAGVenus_EXRC
CAGVenus_EXRC1

Xla.Tg(actc1:GFP)Mohun

NXR_0007

Description: (Cardiac actin:GFP) 580bp of X. laevis actc1b gene driving GFP expression. Embryonic striated muscle including heart.

Reference: Dev Biol. 2002 May;5:57-70 | Development. 2003 Aug;130(16): 3865-76XenbaseEXRC

CardiacActinGFP

Xla.Tg(CMV:GFP)Murray

NXR_0008

Description: (CMV-GFP) CMV promoter driving GFP expression, Whole embryo green fluorescence.

Reference: Xenbase

CMV-GFP_1EXRC
CMV-GFP_2EXRC

Xla.Tg(CMV:GFP,dsRed)Amaya

NXR_0009

Description: Initial ubiquitous green fluorescence switches to red fluorescence following FLP recombinase activation. The constitutive simian cytomegalovirus CMV IE94 promoter/enhancer driving GFP flanked by FRT sites (for FLP site specific recombination). After FLP recombination, CMV drives expression of dsRed.

Previous name: Xla.Tg(CMV:GFP,RFP)Amaya

Available as frozen sperm, please allow 8 months for live animals.

Reference: Gene. 1996 Nov;180:225-7 | Nucleic Acids Res. 2001 Jun;29:E53-3 | Nucleic Acids Res. 2003 Apr;31:e44XenbaseEXRC.

CMVGFPRFP_1EXRC
CMVGFPRFP_2EXRC
CMVGFPRFP_3EXRC

Xla.Tg(Xtr.col2a1:GFP;cryga:GFP)Hank

NXR_0010

Description: (Col2a1-GFP) X. tropicalis collagen 2α1 promoter and first intron driving eGFP, γ-cry driving GFP marker. GFP expression in cartilaginous skeleton.

Reference: Int J Dev Biol. 2010;54(1):141-50 | XenbaseEXRC

Col2a-GFPassembled

Xla.Tg(eef1a1:GFP;cryga:GFP)Krieg

NXR_0011

Description: (Ef1α-GFP) Eef1a1 gene driving GFP expression, γ-cry driving GFP marker. Widespread  GFP expression apparent from mid-gastrula on.

Available as frozen sperm, please allow 8 months for live animals.

Reference: Gene. 1994 Sep;147:223-6Xenbase

ef1aGFP_5

Xla.Tg(CMV:memGFP,cryga:mCherry)NXR

NXR_0012

Description: (CMV:memGFP) CMV promoter driving GFP in cell membrane, γ-cry driving mCherry marker. Ubiquitous membrane GFP expression and mCherry in lens.

Reference: Custom transgenic generated by the NXR. Xenbase

mem-GFP-transgenic-images

Xla.Tg.(Rno.elas:GFP)Jmws

NXR_0013

Description: (Elas-GFP) Rat elastase promoter driving GFP. GFP expression in the pancreas.

Reference: Mech Dev. 1999 Nov;88:221-7 | Development. 2009 Jun;136:1791-800 | Dev Biol. 2007 Apr;304(2): 786-99 | Curr Biol. 2003 Jan;13(2):105-15 | Xenbase.

bro-1-gfp-bright-pancreas

Xla.Tg(CAG:KikGR)Flc

NXR_0014

Description: (CAG-KikumeGR) CAG promoter driving green-to-red photoconvertible fluorescent protein, Kikume Green-Red (KikGR)

Reference: BMC Development Biology 2009 Sept; 9:49 | Development. 2013;140:2409-2421Xenbase

KikumeGFPEmbryo
KikumeGFP-RFPEmbryo

Xla.Tg(cryga:RFP;CMV:HyPer-YFP)Amaya

NXR_0016

Description: (HyPer) CMV promoter driving YFP of H2O2 indicator, HyPer. γ-cry driving RFP. Ubiquitous HyPer YFP expression, marker for integration selection; RFP expression in lens.

Reference: EXRCXenbase

Hyper_2
hyper_1

Xla.Tg(Dre.noto:GFP)1Amaya

NXR_0017

Description: (FLH-GFP) Zebrafish flh promoter driving GFP in the pineal gland and posterior notochord.

Reference:  Concha, M., et. al. (2003) Local tissue interactions across the dorsal midline of the forebrain establish CNS laterality. Neuron; 39(3):423-38XenbaseEXRC

FLH-GFP_EXRC

Xla.Tg(kdr:GFP)Mead

NXR_0018

Description: (FLK1-GFp) 2.5kb X. laevis flk-1 promoter driving GFP. GFP expression in vascular endothelial cells.

Reference: Mead Lab | Dev Dyn. 2007 Oct;236:2808-17XenbaseEXRC

KDRGFP
FLK1GFP_1
FLK1GFP

Xla.Tg(gata1:GFP)Mead

NXR_0019

Description: (GATA1-GFP) GATA-1 promoter and first intron driving expression of GFP, sleeping beauty. GFP expression in red blood cells.

Reference: Mead Lab. XenbaseEXRC

gata1_4
gata1gfp

Xla.Tg(cryga:GFP;hsp70-nog)Jmws

NXR_0020

Description: (HGEM-Noggin) Lenses glow green; heat-shock inducible Noggin.

Reference: Dev Cell. 2003 Sep;5:429-39Mech Dev. 2006 Sep;123:674-88XenbaseEXRC

HGEM_Noggin

NXR_0021

Description: (HGEM-Xtdkk1) Heat-shock inducible Dkk1 to down-regulate Wnt/B-catenin signaling with a Gamma crystallin GFP selection marker.

Reference: Dev Biol. 2008 Apr 15;316(2):323-35Xenbase.

HGEM_DKK1

Xla.Tg(slurp1l:GFP)Mohun

NXR_0025

Description: (LURP1-GFP) 5kb of X.laevis slurp1l gene driving eGFP. Embryonic macrophages, definitive myeloid blood cells, restricted neural expression, epidermis.

Reference: Mech Dev. 2002 Sep;117:173-86Dev Growth Differ. 2006 Oct;48:499-512XenbaseEXRC

LURP1GFP
LURP1GFP_1

Xla.Tg(myl3:eGFP)Mohun

NXR_0026

Description: 8kb of X. laevis myl3 gene driving enhanced GFP. GFP expression in heart mycoardium.

Previous names: MLC1V_GFP, mlc3:eGFP

Reference:  Dev Dyn. 2005 Apr;232(4):1003-12XenbaseEXRC

myl3_2
myl3_3-150x150

Xla.Tg(myl2:eGFP)Mohun

NXR_0027

Description: (MLC2-GFP) 3kb of X. laevis myosin light chain promoter driving eGFP. eGFP expression in ventricular charmber, heart myocardium, and interhyoid facial muscle.

Reference: Development. 2004 Feb;131(3):669-79XenbaseEXRC.

Xla.Tg.(nkx2-5:GFP)Mohun

NXR_0030

Description: (Nkx2.5-GFP) 8kb promoter of X. laevis nkx2-5 gene driving GFP expression. Early heart field, mesoderm, pharyngeal, endoderm, spleen.

Reference: Dev Biol. 2000 Nov 1;227(1):65-79XenbaseEXRC.

Nkx2.5_2Embryo
Nkx2.5_1Embryo
Nkx2.5Embryo

Xla.Tg(hsp70:CActnnb1-GFP;Rno.elas:GFP)Jmws

NXR_0033

Description: (pHS-bCatenin-GFP:Elas-GFP) Generated via REMI (without restriction enzyme) with a cassette containing heatshock promoter driving expression of N-term truncated beta-catenin (stabilized CA form) tagged with GFP. Presumably the cassette also included elas:GFP but that is not described in the paper. The original vector from which pHsβcat∗GFP was derived was named pCH85.

Reference: Developmental Biology, 2008, Sept 15; 316(2): 323-335

bCat-Elas-GFPassembled

Xla.Tg(smad3:eGFP;cryga:GFP)Mohun

NXR_0034

Description: (SMAD-GFP) 1.5kb of X. laevis Smad3 gene driving eGFP. Dorsal mesoderm post gastrulation, heart endocardium, widespread expression at larval stages.

Reference: Mech Dev. 2011 May-Jun;128:303-15XenbaseEXRC.

Smad3-Ventral1
Snap-166

Xla.Tg(tubb2b:mapt-GFP)Amaya

NXR_0035

Description: (NBT/Tubb2-GFP) 3481bp  promoter and 5’UTR of tubb2b driving expression pf mapt-fusion. Green fluorescence in neaural tissue.

Reference: XenbaseEXRC.

Tubb2GFP
TUBB2GFP

Xla.Tg(cryga:eGFP)Amaya

NXR_0036

Description: (xCry-GFP) The crystalline –GFP construct was generated by sub-cloning 2.2 kb a fragment of the crystalline genomic DNA encompassing the promoter region (received from Robert Grainger), upstream of an enhanced version of the GFP3.

Reference: Gene. 1993 Jun;128:189-95XenbaseEXRC.

crygaGFP_EXRC

Xla.Tg(CMV:eYFP,eCFP)1Ryff

NXR_0040

Description: (Y2) Whole embryo blue fluorescence; yellow fluorescence upon Cre recombination.

Reference: Nucleic Acids Res. 2003 Apr 15;31:e44XenbaseEXRC

Y2CMVGFP

Xla.Tg(Xtr.foxi1:katushka;cryga:Venus)EXRC

NXR_0047

Description: (Foxi1-Katushka) 2000bp X. tropicalis foxi I promoter driving expression of Katushka, γ-cry driving Venus marker. Katushka expression in epidermal cells and Venus in lens as integration marker.

Reference: EXRC.

Foxi1-Katushka_1EXRC

Xla.Tg(pax3:Katushka;cryga: Venus)EXRC

NXR_0049

Description: (Pax3-Katushka) 2916bp X. laevis Pax3 promoter driving expression of Katushka in the neural ectoderm (neural crest).

Reference:  Pax3 promoter from Roberto Mayor (Mauricio Moreno). XenbaseEXRC

pax3katushka3
pax3katushka_1

Xla.Tg(Xtr.tbxt:Katushka;cryga:Venus)EXRC

NXR_0050

Description: (Brachyury-Katushka) 3856bp X. tropicalis Brachyury promoter driving expression of Katushka, γ-cry driving Venus marker. Katushka expression in muscles post-gastrulation and Venus in lens as integration marker.

Reference: XenbaseEXRC.

Brachyury-Katushka1EXRC
Brachyury-Katushka2EXRC

Xla.Tg(Xtr.cdknx:GFP)Papal

NXR_0051

Description: (Xic-GFP) 4.7 kb X. tropicalis  p27 Xic1 promoter driving GFP expression in the neural tube and the eye.

Available as frozen sperm, please allow 8 months for live animals.

Reference: Mech of Dev. 120; 607–616XenbaseEXRC

XicGFP_1Embryo
XicGFPEmbryo
XicGFP_2Embryo

Xla.Tg(Rno.tuba1a:GFP)Brown

NXR_0053

Description: (TubulinA1-GFP) Rat tubulin a-1 promoter fused in grame to GFP. GFP expression in the nervous system and later in growing limbs.

Reference: PNAS 96, 14389-14393XenbaseEXRC.

TubulinA1GFP_EXRC

Xla.Tg(ins:memGFP,cryga:mCherry)Brown

NXR_0056

Description: (Insulin-memGFP) Laevis 5kb insulin promoter driving memGFP expression; gamma crystallin mCherry selection

Reference: Transgenic generated by Donald Brown. Xenbase

male-4-bro-2-mid-gut-zoom
gfp-bf-head

Xla.Tg(CMV:eGFP-OMP25)Wtnbe

NXR_0059

Description: (Dria-GFP) CMV driving eGFP tagged OMP25 transmembrane segment. GFP expression of mitochondria membranes.

Reference: Dev Growth Differ. October 1, 2012; 54 (8): 767-76Xenbase

Dria-GFP_1Embryo
Dira-GFP_3Embryo
Dria-GFPEmbry

Xla.Tg(tubb2b:GFP,Aequorin)NXR

NXR_0062

Description: (Tubb2-GFP-Aequorin)

Reference: Custom transgenic generated by the NXR for Andrew Miller

Xla.Tg(CMV:Aequorin,GFP)NXR

NXR_0063

Description: (CMV-Aequorin-GFP) Aequorin is a Ca2+ dependent bioluminescence photoprotein from hydrozoan A. Displays an emission peak at 470nm (blue light).

Reference: Custom transgenic generated by the NXR for Andrew Miller. Xenbase.

Xla.Tg(WntREs:dEGFP)Vlemx

NXR_0064

Description: (pbin7LEF-GFP/Wnt Reporter) Construct contains a synthetic Wnt-responsive promoter – consisting of 7 copies of a TCF/LEF1 binding DNA element, a minimal TATA box and a reporter gene encoding destabilized EGFP and a polyA sequence. The transgene is flanked on both sides by two copies of the chicken HS4-core sequence. GFP expression of dynamic patterns of Wnt/β-catenin signalling activity.

Reference: Proc Natl Acad Sci USA. 2010 Sep 14; 107(37): 16160-16165XenbaseEXRC

pbin8LEFGFP

Xla.Tg(rho:Eco.nfsA)Zuber

NXR_0067

Description: (XOPNTR) Rod optsin promoter driving E. coli nitroreductase.

Reference: FEBS Lett. 1998 Feb;423:117-21Invest Ophthalmol Vis Sci. 2011 Jan;52:364-73XenbaseEXRC.

Xla.Tg(Mmu.tal1:GFP)Patnt

NXR_0069

Description: (SCL-GFP) Mouse SCL +19 enhancer which contains a GATA-ETS-GATA core motif driving GFP. Expression in haematopoietic precursors.

Reference: EMBO J. 2002 Jun 17;21(12):3039-50Mol Cell Biol. 2010 Dec;30(24):5741-51XenbaseEXRC.

sclgfp4

Xla.Tg(tuba1a:MyrPalm-mRFP1)NXR

NXR_0074

Description: (AlphaTubulin-UTR-RFP) tubulin alpha 1a promoter driving expression of membrane bound red fluorescent protein primarily in the ciliated epidermal cells.

Reference: Custom transgenic generated by the NXR for John Wallingford. Xenbase

alphatubulinutrrfp_2
alphatubulinutrrfp

Xla.Tg(CMV:vsFP_Butterfly1.2)NXR

NXR_0092

Description: (CMV-Butterfly VSFP1.2) VSFP is voltage sensitive fluorescence protein. VSFP-Butterfly 1.2 enables optical recordings at high spatial resolution with intrinsic assignment of recorded signals to VSFP-targeted (neural) cell populations.

Reference: Custom transgenic generated by the NXR for Mike Levin. Xenbase

Xla.Tg(CMV:hist2h2be-RFP;cryga:Venus)NXR

NXR_0094

Description: (CMV-H2BmRFP) CMV promoter driving RFP in nuclear membrane, γ-cry driving Venus marker. Ubiquitous nuclear RFP expression, and Venus expression in lens.

Reference: Custom transgenic generated by the NXR. Xenbase

CMVH2BRFP_1Emrbryo
CMVH2BRFP
CMVH2BRFPEmbryo

Xla.Tg(CMV:vsFP2.42_SRL)NXR

NXR_0095

Description: (CMV-VSFP2.42-SRL) vsFP is a voltage sensitive Fluorescent Protein. vsFP enables optical recordings at high spatial resolution with an intrinsic assignment of recorded signals to vsFP-targeted (neural) cell populations.

Reference: Custom transgenic generated by the NXR for Mike Levin. Xenbase

Xla.Tg(CMV:cetn4-RFP)NXR

NXR_0100

Description: (Centrin-RFP) CMV promoter driving RFP and (cetn4) fused with RFP. RFP expression in the microtubules of cilia/ciliated cells.

Reference: Custom transgenic generated by the NXR for Brian Mitchell. Xenbase

Xla.Tg(Dre.cdh17:eGFP)NXR

NXR_0102

Description: (Cdh17inteGFP) Zebrafish(Dre) Li-cadherin (CDH17) driving eGFP. GFP expression in the pronephros/pronephric tubes.

Reference: Custom transgenic generated by the NXR for Rachel Miller. Genes 2018, 9(4)Xenbase

F2_St.47_1
Cdh17inteGFP
F2_St.472

Xla.Tg(tuba1a:deup1-eGFP)NXR

NXR_0103

Description: (Deup1-GFP) Tubulin promoter driving Deup1 fused to eGFP. eGFP expression of deuterosomes and centrioles specifically in multi-ciliated cells. Marker for visualizing centrioles.

Reference: Custom transgenic generated by the NXR for Brian Mitchell. Xenbase.

Deup1-GFP
Deup1-GFP_2

Xla.Tg(Mmu.prrx1:GFP3)Tamur

NXR_0106

Description: (mPrx1-GFP) GFP labels mesenchymal cells in limb bud and limb blastema.

Reference: EXRCDev Biol 304 (2007) 675–686Xenbase

mPrx1

Xla.Tg(tubb2b:GCaMP6s,Rno.elas:GFP)NXR

NXR_0107

Description: (ElasGFP:Tubb2-GCaMP6s) Neaural expression of the green Ca2+ sensor, GCaMP6s, and expression of GFP in the pancreas

Reference: Custom transgenic generated by the NXR. Xenbase

GCamp6s_male1gif
GCaMP6s_male1

Xla.Tg(sox10:GFP)Jpsj

NXR_0108

Description: (Sox10-GFP) Sox10:GFP-HS4-Isce1 transgene 4.7kb upstream from sox10 transcription factor. GFP expression in the migratory cranial neural crest cells.

Reference: EXRCXenbase.

Sox10GFP-st33-Sokol
Sox10GFP-st26-Sokol
Sox10GFP-st23-Sokol

Xla.Tg(CMV:Hsa.EB3-eGFP)Ueno

NXR_0109

Description: (EB3-GFP) CMV driving eGFP of human EB3. Ubiquitous expression of eGFP in microtubule plus ends

Reference: EXRCTakagi et al. 2013 Dev. Growth Differ 55, 422-433Xenbase

EB3GFP

Xla.Tg(CMV:Hsa.B4GALT1-eGFP)Ueno

NXR_0110

Description: (GFP-Golgi) CMV driving GFP of hb4GalT transgene. Ubiquitous expression of eGFP in the golgi appartus.

Reference: EXRCTakagi et al. 2013 Dev. Growth Differ 55, 422-433. Xenbase

EGFPGolgi-e1497379635319
EGFP-Golgi_1EXRC
EGFP-Golgi_2EXRC

Xla.Tg(hsp70:eYFP)Ryff

NXR_0111

Description: (HSP Cre1(C5)) Cross of HSP:Cre1 and C5, heat shocked in embryogensis and EYFP positive tadpoles were grown.

Reference: EXRCXenbase

Xla.Tg(CMV:calr-RFP)Ueno

NXR_0113

Description: (RFP-ER) CMV promoter driving RFP in CALR targeting sequence. Ubiquitous expression of RFP in the endoplasmic reticulum.

Reference: EXRCTakagi et al. 2013 Dev. Growth Differ 55, 422-433Xenbase.

RFPER
RFPER_2EXRC
RFPER_1EXRC

Xla.Tg(Xtr.lmo2:Katushka;cryga:Venus)EXRC

NXR_0114

Description: (LMO2-Katushka) 4775bp X. tropicalis LMO2 promtoer driving Katushka, γ-cry driving VenusGFP marker. Blood marked with red fluorescent protein Katushka, and Venus YFP variant expressed in the lens.

Reference: EXRCXenbase

head-ventral-side-red-plus-2_-bright-4s-exposure
whole-bro-red-8s-exposure

Xla.Tg(CMV:RFP-CAAX)Ueno

NXR_0115

Description: (RFP-Mem) CMV promoter driving RFP in cell membrane. Ubiquitous membrane RFP expression.

Reference:  EXRCTakagi et al. 2013 Dev. Growth Differ 55, 422-433Xenbase

RFPmem

Xla.Tg(Dre.mpeg1:eGFP;cryga:mCherry)Amaya

NXR_0118

Description: (mpeg-EGFP,crygamCherry) Zebrafish mpeg promoter driving GFP, γ-cry driving mCherry marker. GFP expression in the macrophages and mCherry in the lens.

Reference: EXRC | Ellett, F. et. al. (2011) “Mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish”. Blood; 117(4):e49-56Xenbase

mpegEGFPcrymCherry

Xla.Tg(cryga:mCherry;CMV:6xMyc-EosFP)NXR

NXR_0119

Description: (CMV:EosFP)

Reference: Custom transgenic line generated by the NXR for Michael Levin

CMVEOSFP_red
CMVEOSFP_green

Xla.Tg(cryga:mCherry;CMV:zFucci)NXR 

NXR_0120 

Description: (CMV:zFucci) 

Reference: Custom transgenic line generated by the NXR for Michael Levin 

Xla.Tg(hsp70:Cre)Ryff

NXR_0121

Description: (Hsp:Cre1) Cre recombinase drive by HSP70

Reference: EXRCRoose,M., et. al. (2009) “Heat-shock inducible Cre strains to study organogenesis in transgenic Xenopus laevis.” Trans Res. 18, 595-605Xenbase

Xla.Tg(rho:eGFP-atp1a3)Baker

NXR_0122

Description: (eGFP-ATP1a3) Xenopus rhodopsin promoter driving expression of eGFP tagged neuronal α3 isoform of the Na+/K+-ATPase in the rod photoreceptors.

Referenece: Traffic. 2015 Dec;16(12):1239-53Xenbase.

positive-bro-1

Xla.Tg(Hsa.UBC:Gal4-VP16;cryga:CFP;UAS:mCherry-msn;UAS:Venus-mapt)Amaya

NXR_0123

Description: (Ubi-Gal4:UAS-venusTau:UAS-mCherryMoe)

Ubiquitous red fluorescence marking F-actin and green fluorescence marking microtubules. Cyan fluorescence marking the eye lens. Line was generated by digestion of three plasmids with Asp718 and then co-injection via REMI. First plasmid: 1.5 kb of human ubiquitin C promoter driving expression of Gal4-VP16 and gamma crystalin promoter driving expression of CFP. Second plasmid: UAS driving expression of mCherry tagged moesin. Third plasmid: UAS driving expression of Venus tagged microtubule associated protein tau.

Reference: EXRCXenbase

ubiqgal4uasvenustauuasmcherrymoe

Xla.Tg(cryga:mCherry;CMV:6xHis-CaMPARI)NXR

NXR_0124

Description: (CMV:CaMPARI)

Reference: Custom transgenic line generated by the NXR for Michael Levin

CMVCaMPARI_male2xcry
CMVCaMPARI_male2gfp

Xla.Tg(tubb2b:ChIEF,Rno.elas:GFP)NXR

NXR_0125

Description: (Elas:GFP,Tubb2bChIEF) Neaural expression of blue light photoinducible channelrhodopsin, ChIEF, and expression of GFP in the pancreas.

Reference: Custom transgenic generated by the NXR. Xenbase

ChIEF

Xla.Tg(hsp70:DNyap1-GFP,cryga:tdTomato)Tamur

NXR_0126

Description: (HSP70:dnYAP-2A-GFP,gCry:tdTomato) 330 bp of promoter of the X. laevis hsp70 gene driving the expression dominant negative form of Yap (dnYap) tagged with GFP via 2A peptrides of 2.2 kb gamma crystalline promoter driving tdTomato.

Reference: EXRCXenbase

hspdnyap_exrc

Xla.Tg(Hsa.UBC-Gal4;UAS:HyPer-YFP)Amaya

NXR_0127

Description: Detects hydrogen peroxide in vivo. Ubiquitous expression of YFP in tissues with reactive oxygen species (ROS).

Reference: EXRCXenbase

head-top-1-photoshop
tail-somites-1-photoshop
head-side-1-photoshop

Xla.Tg.(hsp70:DNfgfr-Venus:cryga:RFP)Amaya

NXR_0128

Description: (HSP-XFDvenus; cryRFP) HSP70 promoter driving dominant negative FGFR with crystallin-RFP.

Reference: EXRCXenbase

Xla.Tg(Hsa.UBC:COX8A-dsRed;cryga:GFP)EXRC

NXR_0129

Description: (Ubic dsRed-Mito) 1.5kb of UbiC promoter driving dsRed and COX8, γ-cry driving Venus marker. Ubquitous dsRed expression in mitochondria, and Venus in lens.

Reference: EXRC. Xenbase

Katushka-Mito1

Xla.Tg(CMV:H3K9ac-eGFP)Ueno

NXR_0130

Description: (Mintbody/CMV-H3K9ac-EGFP) CMV promoter driving eGFP of h3k9ac transgene. Ubiquitous GFP histone expression.

Reference: Genes Cells. 2016 21:358–369Sci Rep. 2013; 3:2436Xenbase

UenoMintbody1
UenoMintbody
UenoMintbody3
UenoMintbody2

Xla.Tg(pax8:GFP)Ogino

NXR_0132

Description: (Pax8-GFP) Pax8 driving GFP. GFP expression of kidney and head.

Reference: Nat Commun. 2012 May 22;3:848XenbaseEXRC.

pax8gfp_1
pax8gfp_3

Xla.Tg(CAG:SCAT3)Ueno

NXR_0134

Description: (NIBB6/Scat3) CAG-seCFP-Venus (an enhanced yellow fluorescent protein with efficient maturation property), containing the fluorescence indicator molecule SCAT3 and the rabbit β-globin polyA signal sequence, under control of the CAG promoter. SCAT3 is a hybrid protein of seCFP-Venus linked with the caspase-3 recognition sequence (DEVD) in pCAGGS.

Reference: Kominami, K. et al., 2006 Genes Cells 11:701-711Takagi C. et al. 2013 Dev. Growth Differ 55, 422-433XenbaseEXRC

Nibb6_2

Xla.Tg(Rno.elas:rtTA;TRE:notchICD;cryga:GFP)Brown

NXR_0135

Description: (Elas-rtTA25-M2/tet(O)-NICD,cryGFP) Tetracycline inducible activated Notch expression in the exocrine pancreas.

Reference: Dev. Biology. 2011 351:2, 311-317Xenbase

elaseta25mztetonicd3

Xla.Tg(CMV:hist2h2be-GFP;CMV:mRFP)Flc

NXR_0139

Description: (CMV:H2B-GFP;CMV:mRFP) Cross of CMV:mRFP parent created CMV IE94 promoter in pCS2+ backbone, RFP inserted at BstBI and linearized with NotI; and CMV:H2B-GFP parent. Ubiquitous GFP expression of nuclear membrane and ubiquitous RFP expression of cell membrane.

Reference: Conlon LabXenbase

h2bgfpmemrfp_4
h2bgfpmemrfp_4rfp
h2bgfpmemrfp_3
h2bgfpmemrfp_3rfp

Xla.Tg(xtr.HAL2:GFP;cryga:GFP)Shi

NXR_0142

Description: (PDP-HAL2:EGFP) This transgenic was developed in Yun-Bo Shi's lab. Construct contains a 4.4 kb Xenopus tropicalis HAL2 promoter driving the expression of enhanced green fluorescent protein (EGFP) in the intestinal epithelium at metamorphosis.  The transgenic construct also contains a gamma-crystallin promoter driving the expression of GFP in the eyes of the animals.

Reference: Endocrinology 158(4):1022-1033.

Xla.Tg(hsp:F-dpTR;cryga:GFP)Shi

NXR_0143

Description: (PCGH-dpTR) This transgenic was developed in Yun-Bo Shi's lab. The Construct is a double promoter construct containing a gamma-crystallin promoter driving the expression of GFP, and a heat shock-inducible promoter driving the expression of a dominant positive thyroid hormone receptor (F-dpTR).  F-dpTR has a FLAG tag and VP16 activation domain fused to the N terminus of TR and the receptor-interacting domain (RID) of Xenopus N-CoR fused to the C terminus.

Reference: Buchholz et al, MCB 24(20):9026-9037, 2004.

Xla.Tg(CMV:GFP;cryga GFP3)Shi

NXR_0144

Description: (PDP-CMV:GFP) This transgenic was developed in Yun-Bo Shi's lab. The transgenic construct is a double promoter construct containing the gamma-crystallin promoter driving the expression of GFP3, and a CMV promoter driving the expression of GFP.

Reference: 

Xla.Tg(hsp:st3-GFP;cryga:GFP)Shi

NXR_0145

Description: (PCGHSWG) This transgenic was developed in Yun-Bo Shi's lab. The construct is a double promoter construct containing a gamma-crystallin promoter driving the expression of GFP3, and a heat shock-inducible promoter driving the expression of Stromelysisin 3 (ST3) fused to GFP.  GFP3 is a different version of GFP which contains several amino acid substitutions.

Reference: Fu et al, JBC 280(30):27856-27865, 2005.

Xla.Tg(islet2b:mCherry)Marsh-Armstrong

NXR_0146

Description: (islet2b:mCherry)

Reference: 

Xla.Tg(islet2b:GFP)Marsh-Armstrong

NXR_0147

Description: (islet2b:GFP)

Reference: 

Xla.Tg(islet2b:rpl10a-GFP)Marsh-Armstrong

 

NXR_0148

Description: (islet2b:rpl10a-GFP) 

Reference: 

Xla.Tg(hsp70:histh2b-mCherry;2A GFP,cryga:GFP)Henry

NXR_0149

Description: (hsp70:H2BmCherry,2A GFP, cryga:GFP

Reference: DOI: 10.1002/dvg.23411

Xla.Tg(CMV:rtTA;TRE:eGFP;cryga:GFP3)Buchz

NXR_0163

Description: (pDPCrtTA-TREG-HS4) Dox-activated transcription factor rtTA under the control of the ubiquitous promoter CMV and green fluorescent protein GFP is regulated by the modified tetracycline response element TREtight. Construct also has a crystallin:GFP expression cassette for constitutive expression of GFP in the lens as a marker of transgenesis and two directly repeated HS4 insulator elements and I Sce-I sides.

Both CMV:rtTA and TRE:GFP cassettes were included on the same transgenesis construct so expression levels and uniformity of Dox-induced GFP in the F0 tadpoles could be evaluated. (Line and description sourced secondarily from EXRC)

Reference: 2019 Oct;57(10):e23329. DOI: 10.1002/dvg.23329. Epub 2019 Aug 12.

Xla.Tg(col2a1:rtTA;TRE:Cre;cryga:GFP3)Buchz

NXR_0164

Description: (pDPcol2.3rtTA-TRECre-HS4) Line made by coinjecting 3 separate constructs in an I Sce-I reaction.

1. Chondrocyte-specific regulatory elements from the 5’ region and first intron of X.tropicalis col2a1 driving the tetracycline/doxycycline conditional transcription factor rtTA.

2. Tetracycline/doxycycline response element TRE driving Cre recombinase

3. Crystallin gamma A driving GFP as a marker for integration.

(Line and description sourced secondarily from EXRC)

Reference: 2019 Oct;57(10):e23329. DOI: 10.1002/dvg.23329. Epub 2019 Aug 12.

 Kerney, R., et. al. (2012) “Cartilage on the move: Cartilage lineage tracing during tadpole metamorphosis.” Dev. Growth, 54:739-752

Xla.Tg(tubb2b:Katushka;cryga:Venus)EXRC

NXR_0165

Description: (NBT-Katushka γ-cry-VenusGFP /NBT-Katushka (2x ISce-1)) 3481bp of tubb2b (N-β-tubulin, NBT) promoter and 5’UTR driving expression of Katushka (TurboFP-C); marker for integration selection: γ-cry Venus. (I Sce-I). Line and description sourced from EXRC (Line 202)

Reference: Huang, J. K., Dorey, K., Ishibashi, S. and Amaya, E. (2007). “BDNF promotes target innervation of Xenopus mandibular trigeminal axons in vivo.” BMC Dev. Biol. 7, 59.